What is baseline drift

How to choose a metal scavenger? How to purify your API from organic contaminants? What is a supported scavenger? What quantity of scavenger should be used? At what temperature should the scavenger be used? What solvents should be used with our scavengers? At what pH should the scavenging in aqueous solution be performed? Reaction time vs scavenger quantity What is the thermal stability of the SiliaMetS? What is the molecular loading and how do you determine the molecular loading of a SiliaMetS metal scavenger?

Why should I use a guard column with my analytical or preparative column? Ideal injection volume vs column dimension What happens if my sample solvent is stronger than my mobile phase? Can I get a sharper peak by injecting my sample in a weaker injection solvent? How much sample can I inject on my LC column? What size threads are on the end fittings of my HPLC column? Why do most users privilege PEEK vs stainless steel fittings and tubing?

What is the internal diameter of my LC tubing? How can the column performance be checked? How can the columns be cleaned or regenerated? What is the appropriate flow rate for the different column dimensions? What is the difference between dead volume and dwell volume? What is the shipping solvent for your HPLC columns? When should I use a gradient? What are the common buffers used in HPLC?

Why is it difficult to get reproducible retention times in normal phase chromatography? Solution: Use guard column. If necessary flush column with strong solvent between injections or periodically during analysis. Mobile phase recycled but detector not adjusted. Solution: Reset baseline. Use new materials when dynamic range of detector is exceeded. Detector UV not set at absorbance maximum but at slope of curve. Solution: Change wavelength to UV absorbance maximum.

Solution: Higher temperatures can enhance the polymerization of ACN resulting in building of polymers. We develop and manufacture scientific instruments of superior quality for liquid chromatography systems and components, including:. We are an established partner in science.

Today and in the future. Home How to deal with a baseline drift? Polluted, degraded or unprepared mobile phases of high-quality chemicals. Strongly preserved materials in the sample can be elucidated as very broad peaks which tend to be rising baseline.

The gradient analysis can make the problem worse. Detector UV not mounted at the highest absorbance but the curve angle. Control procedure of Baseline Drift in HPLC Use a heat exchanger before the detector to control the temperature of the column and mobile process. Use HPLC-grade solvents, high-purity salts, and additives. Mobile process degasses before use, store with helium during use. Flush cell with methanol or other potent solvents.

Unplug or replace the row. Refer to the Window Replacement Detector Manual. To prevent problems, regularly track the composition and flow rate.